Journal: Science Advances

Article Title: Biofunctional lipid nanoparticles for precision treatment and prophylaxis of bacterial infections

doi: 10.1126/sciadv.adk9754

Figure Lengend Snippet: ( A ) Expression levels of CD80 + and CD86 + in BMDCs (CD11c + ) and ( B ) production of IL-6 and TNF-α of the cell supernatant measured by ELISA after different treatments (concentration, 2 μg ml −1 NPs) for 24 hours ( n = 3). ( C ) Schematics of experimental design to evaluate the in vivo short-term immune responses activated by a single dose of various LNPs (20 μg/20 g) via subcutaneous injection. ( D ) Percentage of CD80 + and ( E ) CD86 + cells (gated on CD11c + cells) in the LNs at day 3 after immunization ( n = 6). ( F ) Percentage of CD3 + T cells, ( G ) CD8 + T cells (gated on CD3 + T cells), and ( H ) CD4 + T cells (gated on CD3 + T cells) in the spleen at day 3 after immunization ( n = 6). Naive mice without immunization were used as control. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Fluorescein isothiocyanate (FITC)–conjugated anti-mouse CD11c, phycoerythrin (PE)–conjugated anti-mouse CD80, PE-conjugated anti-mouse CD86, allophycocyanin (APC)–conjugated anti-mouse CD3, PE-conjugated anti-mouse CD8a, and FITC-conjugated anti-mouse CD4 were purchased from Tonbo Biosciences, USA.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay, In Vivo, Injection, Control

Journal: Materials Today Bio

Article Title: A MnO 2 -based tumor-seeking nanoplatform for enhanced chemoimmunotherapy against 4T1 breast cancer

doi: 10.1016/j.mtbio.2025.102000

Figure Lengend Snippet: In vitro BMDC Maturity and BMDM Repolarization Assay. Representative flow cytometry graph of CD80 + CD86 + cells in BMDCs after treated with normal saline (A), PTX injections (B), Squ@APS-IR820 NPs (C), MnO 2 @APS-IR820 NPs (D), Squ@APS NPs + MnO 2 @APS NPs (E), and Squ@APS-IR820 NPs + MnO 2 @APS-IR820 NPs (F). Percentages of CD80 + CD86 + cells (G). Flow cytometry chart of CD86 + CD206 - cells (H–M) and quantification of the proportion (N) of CD86 + CD206 - BMDMs after being incubated with different treatments (n = 3). Differences among groups were calculated by one-way ANOVA analysis. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. ns, no significance.

Article Snippet: Antibody: Anti-mouse CD16/32 Antibody was provided by Elabscience Biotechnology Co., Ltd, Anti-mouse CD11c PE, anti-mouse CD80 FITC, anti-mouse CD86 APC, anti-mouse CD86-PE, anti-mouse CD45-APC was purchased from Proteintech Group, Inc (Hubei, China).

Techniques: In Vitro, Flow Cytometry, Saline, Incubation

Journal: Materials Today Bio

Article Title: A MnO 2 -based tumor-seeking nanoplatform for enhanced chemoimmunotherapy against 4T1 breast cancer

doi: 10.1016/j.mtbio.2025.102000

Figure Lengend Snippet: Immunoassay of the tumor and the spleen after different treatments. (A) Workflow of the immunoassay based on Flow Cytometry. Created in BioRender. Gong, T. (2025) https://BioRender.com/tjhnwh2 . Representative flow cytometry plots of tumor immune cells including CD3 + CD8 + T cells (B), CD11b + Ly6c + MDSCs (C). Representative flow cytometry plots of splenic immune cells including CD80 + CD86 + DCs (D), CD3 + CD8 + T cells (E), and CD3 - B220 + B cells (F). The quantitative analysis of CD3 + CD8 + T cells (G), CD11b + Ly6c + MDSCs (H) in the tumor and CD80 + CD86 + DCs (I), CD3 + CD8 + T cells (J), and CD3 - B220 + B cells (K) in the spleen after different treatments. Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. ns, no significant difference between two groups.

Article Snippet: Antibody: Anti-mouse CD16/32 Antibody was provided by Elabscience Biotechnology Co., Ltd, Anti-mouse CD11c PE, anti-mouse CD80 FITC, anti-mouse CD86 APC, anti-mouse CD86-PE, anti-mouse CD45-APC was purchased from Proteintech Group, Inc (Hubei, China).

Techniques: Flow Cytometry

Journal: PLoS ONE

Article Title: Sulforaphane Epigenetically Regulates Innate Immune Responses of Porcine Monocyte-Derived Dendritic Cells Induced with Lipopolysaccharide

doi: 10.1371/journal.pone.0121574

Figure Lengend Snippet: List of primer sequences used in this study.

Article Snippet: Cells were stained with a mouse anti-human CD40 FITC Ab (clone G28.5, NB100-77786, Novus Biologicals), a mouse anti-human CD80 PE Ab (clone 37711, FAB140P, R&D systems) and a mouse anti-human CD86 APC Ab (clone 37301, FAB141A, R&D systems) antibodies.

Techniques: Amplification

Journal: PLoS ONE

Article Title: Sulforaphane Epigenetically Regulates Innate Immune Responses of Porcine Monocyte-Derived Dendritic Cells Induced with Lipopolysaccharide

doi: 10.1371/journal.pone.0121574

Figure Lengend Snippet: moDCs at day 7 in culture were used for cell phagocytosis and cell differentiation status analysis. moDCs were pre-incubated for 1 h with or without SFN (10 μM) before stimulation for 24 h LPS (1.0 μg/ml) or to the indicated concentrations. CD40, CD80, and CD86 cellular surface markers expression were analyzed by flow cytometry (A). The flow cytometry results shown were from one experiment of two independent experiments. CD40, CD80 and CD86 mean fluorescence intensity (MFI) determined by flow cytometry (B). The flow cytometry results were combined from two independent experiments and each experiment was performed from triplications. Data are mean ± standard deviations (SD) (the letters a and b P< 0.01). The phagocytic activity of moDCs was examined after stimulating with different concentration of LPS (0,5 μg/ml, 1,0 μg/ml, and 2,0 μg/ml) with or without 24 h pre-treatment with SFN (C). The mRNA expression of DCs surface markers CD40, CD80 and CD86 were quantified using qRT-PCR (D). The mRNA expression and phagocytosis results were combined from three independent experiments and each experiment was performed in four replications. The data represented as the mean ± standard deviations (SD) (* P < 0.05; ** P < 0.01; *** P < 0.001).

Article Snippet: Cells were stained with a mouse anti-human CD40 FITC Ab (clone G28.5, NB100-77786, Novus Biologicals), a mouse anti-human CD80 PE Ab (clone 37711, FAB140P, R&D systems) and a mouse anti-human CD86 APC Ab (clone 37301, FAB141A, R&D systems) antibodies.

Techniques: Cell Differentiation, Incubation, Expressing, Flow Cytometry, Fluorescence, Activity Assay, Concentration Assay, Quantitative RT-PCR

Journal: Journal of Cellular and Molecular Medicine

Article Title: TREM2 expression promotes liver and peritoneal M2 macrophage polarization in mice infected with Schistosoma japonicum

doi: 10.1111/jcmm.17842

Figure Lengend Snippet: Trem2 deletion increases F4/80 + CD86+ cells in peritoneal macrophages. (A) TREM2 expression is upregulated in peritoneal macrophages from mice infected with Schistosoma japonicum . The number of TREM2+ cells in F4/80 + CD11b + peritoneal macrophages was determined by flow cytometry and compared to uninfected controls. * p < 0.05, ** p < 0.01, *** p < 0.001. (B) The number of F4/80 + CD86+ cells in total peritoneal macrophages obtained from wild‐type or Trem2 −/− mice with or without IL4/13 stimulation was determined by flow cytometry. ** p < 0.01. (C) The number of F4/80 + CD206+ cells in total peritoneal macrophages obtained from wild‐type or Trem2 −/− mice with or without IL4/13 stimulation was determined by flow cytometry. * p < 0.05. All results are relative to their respective controls.

Article Snippet: For this, the cells were incubated with PE conjugated antibody against mouse F4/80 (02922–60, Biogems), PE‐Cy7 conjugated antibody against mouse CD11b (03221–77, Biogems), APC conjugated antibody against mouse CD86 or CD206 (17–0862, 17–2061, eBioscience) and FITC conjugated antibody against mouse TREM2 (ab119852, Abcam).

Techniques: Expressing, Infection, Flow Cytometry